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Course module: 8PM00
8PM00
Project Molecular biology
Course info
Course module8PM00
Credits (ECTS)5
Category-
Course typeGraduate School
Language of instructionDutch
Offered byEindhoven University of Technology; Biomedical Engineering; Protein Engineering;
Is part of-
Contact personprof.dr. M. Merkx
Telephone4728
E-mailm.merkx@tue.nl
Lecturer(s)
Lecturer
ing. P.T.H.M. de Graaf - Heuvelmans
Other course modules lecturer
Responsible lecturer
prof.dr. M. Merkx
Other course modules lecturer
Contactperson for the course
prof.dr. M. Merkx
Other course modules lecturer
Co-lecturer
dr. C. Ottmann
Other course modules lecturer
Lecturer
S.F.A. Wouters
Other course modules lecturer
Academic year2017
Period
GS1  (04/09/2017 to 12/11/2017)
Starting block
GS1
TimeslotX: no timeslot
Course mode
Fulltime
Remarks-
Registration openfrom 15/06/2017 up to and including 27/08/2017
Application procedureYou apply via OSIRIS Student
Explanation-
Registration using OSIRISYes
Registration open for students from other department(s)Yes
Pre-registrationNo
Waiting listNo
Number of insufficient tests-
Number of groups of preference0
Learning objectives
Goal: development of fluorescent proteins with novel spectral properties using in vitro directed evolution approach. Screening of protein libraries (error-prone PCR or site-saturation mutagenesis) using FACS and characterization of best hits.
Content
Skills and techniques; cloning (restriction analysis, primer design, ligation, PCR, electrophoresis, transformation, DNA extraction, sequencing),Fluorescence Assisted Cell Sorting (FACS), recombinant protein expression and purification, optical bioanalytical techniques reporting in lab notebook, safe microbiological technqiues (VMT).
Entrance requirements
You must have completed the final examination bsc program exam
Entrance requirements tests
-
Assumed previous knowledge
The student is assumed to be familair with the topics taught in Bio-Organic chemistry (8RB10) and Chemical Biology (8RB20).

For ST-studenten Biochemistry (8RA00) is required an either Molecular Cell Biology (8RB00) or Chemical Biology (8RB20)
Previous knowledge can be gained by
-
Resources for self study
-
Short promotional description of the course
Het aanleren van praktische vaardigheden m.b.t. moleculair biologisch onderzoek (VMT certificaat).
Bachelor College or Graduate School
Graduate School
Required materials
-
Recommended materials
-
Instructional modes
Project

General
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Remark
-
Tests
Oral on basis of lab notebook
Test weight100
Minimum grade6
Test type-
Number of opportunities1
OpportunitiesBlock GS1
Test duration in minutes-

Assessment
-

Remark
-

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Kies de Nederlandse taal