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Course module: 8SM10
8SM10
Protein engineering
Course info
Course module8SM10
Credits (ECTS)2.5
Category-
Course typeGraduate School
Language of instructionEnglish
Offered byEindhoven University of Technology; Biomedical Engineering; Protein Engineering;
Is part of-
Contact personprof.dr. M. Merkx
Telephone4728
E-mailm.merkx@tue.nl
Lecturer(s)
Responsible lecturer
prof.dr. M. Merkx
Other course modules lecturer
Contactperson for the course
prof.dr. M. Merkx
Other course modules lecturer
Academic year2017
Period
GS2  (13/11/2017 to 04/02/2018)
Starting block
GS2
TimeslotD: D - We 5-8, Th 9-10, Fr 1-4
Course mode
Fulltime
Remarks-
Registration openfrom 15/06/2017 up to and including 15/10/2017
Application procedureYou apply via OSIRIS Student
Explanation-
Registration using OSIRISYes
Registration open for students from other department(s)Yes
Pre-registrationNo
Waiting listNo
Number of insufficient tests-
Number of groups of preference0
Learning objectives
Proteins are a remarkably versatile class of macromolecules. Enzymes consititute very efficient catalysts for example, but proteins are also involved in molecular recognition (e.g. antibodies) and they form the basis of materials with attractive material properties (collagen, silk).
Content
In this course important methods and techniques (both molecular-biological and chemical) will be discussed that can be used to develop new proteins with new and biomedically attractive properties. The course starts with a renewed introduction to protein structure, followed by some analytical techniques that are important for protein characterization (cicrcular dichrosim, mass spectrometry, X-ray, and NMR). After this general introduction the following subjects will be treated:
  • biological synthesis of proteins (recombinant expression systems, use of affinity tags)
  • chemical synthesis of peptides and proteins
  • chemical modifications of proteins, non-natural amino acids
  • protein folding
  • rational design of new proteins (strategy, site-directed mutagenesis, chimeric proteins)
  • directed evolution and high-throughput library screening
All techniques will be illustrated by giving examples of applications. No specific book is used in this course. The general Biochemistry textbook will be used to treat general aspects of protein chemistry. For specific subjects, review papers will be handed-out. An important part of the course is a seminar that will be organized at the end of the course. In this seminar students will present recent papers on new development in protein engineering and discuss their strengths and weaknesses.
Entrance requirements
You must have completed the final examination bsc program exam
Entrance requirements tests
-
Assumed previous knowledge
• 8RA00 - Biochemistry
• 8RB00 - Molecular cell biology
• 8RB20 - Chemical biology
Previous knowledge can be gained by
-
Resources for self study
-
Bachelor College or Graduate School
Graduate School
Required materials
-
Recommended materials
reader
Instructional modes
Guided selfstudy

General
-

Remark
-
Lecture

General
-

Remark
-
Tests
Written examination
Test weight100
Minimum grade6
Test typeFinal examination
Number of opportunities2
OpportunitiesBlock GS2, Block GS3
Test duration in minutes-

Assessment
Written (50%) en colloquium about a article out of recent literature (50%)

Remark
Re-examination in consultation between the lecturer.

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